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CD40 ligand (CD40L), Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2008
- Product Name CD40 ligand (CD40L), Human, ELISA assay
- Product Description The human CD40L Kit is a sandwich ELISA. The capture antibody is a polyclonal human CD40L antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CD40L concentration are added to these wells and allowed to complex with the bound CD40L antibody. A biotinylated human CD40L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40L present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD40L in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names CD40-L; TRAP; gp39; TRAM; CD154; Tumour necrosis factor ligand superfamily member 5; TNFSF5; CD40L;
- Application(s) ELISA
- Specificity Human CD40L
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids expression system consisting of amino acids, E108-L261
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 15 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human CD40L Kit is a sandwich ELISA. The capture antibody is a polyclonal human CD40L antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CD40L concentration are added to these wells and allowed to complex with the bound CD40L antibody. A biotinylated human CD40L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40L present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD40L in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of CD40 ligand (CD40L) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target CD40 ligand (CD40L)
- Specificity Human CD40L
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids expression system consisting of amino acids, E108-L261
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 15 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names CD40-L; TRAP; gp39; TRAM; CD154; Tumour necrosis factor ligand superfamily member 5; TNFSF5; CD40L;
- Uniprot Number P29965
- Uniprot Number/Name P29965 (CD40L_HUMAN)
- Scientific Background CD40L is the ligand for CD40, a member of the tumour necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.