1800 605-5127
+61 (0)8 8352 7711
Ciliary neurotrophic factor (CNTF), Rat, ELISA assay
As low as
US$547.00
Only %1 left
Catalog Number
BEK-2340
- Product Name Ciliary neurotrophic factor (CNTF), Rat, ELISA assay
-
Product Description
The Rat CNTF ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal CNTF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CNTF concentration are added to these wells and allowed to complex with the bound CNTF antibody. A biotinylated CNTF goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CNTF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CNTF in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Ciliary neurotrophic factor; CNTF;
- Application(s) ELISA
- Specificity Rat CNTF
- Species Reactivity Rat
- Immunogen Description The standard and immunogen for this kit is recombinant Rat CNTF produced in E.coli cells; Immunogen sequence: A2-M200.
- Range 31.2 - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
The Rat CNTF ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal CNTF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CNTF concentration are added to these wells and allowed to complex with the bound CNTF antibody. A biotinylated CNTF goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CNTF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CNTF in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Ciliary neurotrophic factor (CNTF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Ciliary neurotrophic factor (CNTF)
- Specificity Rat CNTF
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and immunogen for this kit is recombinant Rat CNTF produced in E.coli cells; Immunogen sequence: A2-M200.
- Range 31.2 - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), rat CNTF protein standards, Rat CNTF biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Ciliary neurotrophic factor; CNTF;
- Uniprot Number P20294
- Uniprot Number/Name P20294 (CNTF_RAT)
- Scientific Background CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
-
Specific References
Su X et al. (2023) Decellularized extracellular matrix scaffold seeded with adipose-derived stem cells promotes neurorestoration and functional recovery after spinal cord injury through Wnt/ β-catenin signaling pathway regulation Biomed Mater. 19(1). Application: Rat, Tissue homogenate; Cell culture supernatant.