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E-selectin, soluble (sE-selectin), Human, ELISA assay
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Catalog Number
BEK-2089
- Product Name E-selectin, soluble (sE-selectin), Human, ELISA assay
- Product Description The human E-Selectin Kit is a sandwich ELISA. The capture antibody is a monoclonal human E-Selectin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known E-Selectin concentration are added to these wells and allowed to complex with the bound E-Selectin antibody. A biotinylated human E-Selectin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of E-Selectin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human E-Selectin in samples such as culture supernatant, serum, plasma (Citrate) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; SELE; ELAM1; Soluble E-Selectin; sE-Selectin;
- Application(s) ELISA
- Specificity Human E-Selectin
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids W22-P556
- Range 125 pg/mL - 8,000 pg/mL
- Sensitivity < 4 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human E-Selectin Kit is a sandwich ELISA. The capture antibody is a monoclonal human E-Selectin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known E-Selectin concentration are added to these wells and allowed to complex with the bound E-Selectin antibody. A biotinylated human E-Selectin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of E-Selectin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human E-Selectin in samples such as culture supernatant, serum, plasma (Citrate) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of E-selectin, soluble (sE-selectin) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target E-selectin, soluble (sE-selectin)
- Specificity Human E-Selectin
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids W22-P556
- Range 125 pg/mL - 8,000 pg/mL
- Sensitivity < 4 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; SELE; ELAM1; Soluble E-Selectin; sE-Selectin;
- Uniprot Number P16581
- Uniprot Number/Name P16581 (LYAM2_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Bang OY et al. (2018) "Biomarker for Diagnosis of Moyamoya disease." United States Patent Application 20180030535. Application: ELISA, Species: Human.
Bang OY et al. (2016) "Caveolin-1, Ring finger protein 213, and endothelial function in Moyamoya disease." Int J Stroke. [Epub ahead of print]. Application: ELISA, Species: Human.
Bang OY et al. (2015) "Brain Microangiopathy and Macroangiopathy Share Common Risk Factors and Biomarkers" Atherosclerosis In press. Application: ELISA, Species: Human, blood.Biomarker, intracranial atherosclerosis, small artery disease, Risk factor, renal function, Endothelial function