Product DescriptiongoogleThe human Eotaxin Kit is a sandwich ELISA. The capture antibody is a monoclonal human Eotaxin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Eotaxin concentration are added to these wells and allowed to complex with the bound Eotaxin antibody. A biotinylated human Eotaxin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Eotaxin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Eotaxin in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, G24-P97
Product DescriptionThe human Eotaxin Kit is a sandwich ELISA. The capture antibody is a monoclonal human Eotaxin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Eotaxin concentration are added to these wells and allowed to complex with the bound Eotaxin antibody. A biotinylated human Eotaxin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Eotaxin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Eotaxin in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Eotaxin in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetEotaxin
SpecificityHuman Eotaxin
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesHuman
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, G24-P97
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specific ReferencesSugimoto M. & Kondo M. (2016), Lecithin-bound iodine prevents disruption of tight junctions of retinal pigment epithelial cells under hypoxic stress. Journal of Ophthalmology. Volume 2016, Article ID 9292346. Application: Cell culture supernatant.