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Epidermal growth factor (EGF), Mouse, ELISA assay
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US$547.00
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Catalog Number
BEK-2010
- Product Name Epidermal growth factor (EGF), Mouse, ELISA assay
- Product Description The mouse Epidermal Growth Factor (EGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse EGF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known EGF concentration are added to these wells and allowed to complex with the bound EGF antibody. A biotinylated mouse EGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of EGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse EGF in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names epidermal growth factor (beta-urogastrone); HOMG4; Pro-epidermal growth factor; EGF;
- Application(s) ELISA
- Specificity Mouse Epidermal Growth Factor
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, N977-R1029
- Range 7.8 pg/mL - 500 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Epidermal Growth Factor (EGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse EGF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known EGF concentration are added to these wells and allowed to complex with the bound EGF antibody. A biotinylated mouse EGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of EGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse EGF in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Epidermal growth factor (EGF) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Epidermal growth factor (EGF)
- Specificity Mouse Epidermal Growth Factor
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, N977-R1029
- Range 7.8 pg/mL - 500 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum, Urine
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names epidermal growth factor (beta-urogastrone); HOMG4; Pro-epidermal growth factor; EGF;
- Uniprot Number P01132
- Uniprot Number/Name P01132 (EGF_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
