Product DescriptiongoogleThe human Epidermal Growth Factor receptor (EGFR) Kit is a sandwich ELISA. The capture antibody is a polyclonal human EGFR antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known EGFR concentration are added to these wells and allowed to complex with the bound EGFR antibody. A biotinylated human EGFR polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of EGFR present in the samples. The purpose of this kit is the in-vitro quantitative determination of human EGFR in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and human milk. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesEGFR; ERBB1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1;
Application(s)ELISA
SpecificityHuman Epidermal Growth Factor receptor
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids M1-S645
Product DescriptionThe human Epidermal Growth Factor receptor (EGFR) Kit is a sandwich ELISA. The capture antibody is a polyclonal human EGFR antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known EGFR concentration are added to these wells and allowed to complex with the bound EGFR antibody. A biotinylated human EGFR polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of EGFR present in the samples. The purpose of this kit is the in-vitro quantitative determination of human EGFR in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and human milk. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Epidermal growth factor receptor (EGFR) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), Human Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetEpidermal growth factor receptor (EGFR)
SpecificityHuman Epidermal Growth Factor receptor
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesHuman
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids M1-S645
Range156 pg/mL - 10,000 pg/mL
Sensitivity< 1 pg/mL
Sample TypeCulture Supernatant, Human Milk, Plasma (EDTA), Plasma (Heparin), Serum
Detection MethodColorimetric
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesEGFR; ERBB1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1;