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Fms-related tyrosine kinase 3 ligand (Flt3 ligand), Human, ELISA assay
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Catalog Number
BEK-2344
- Product Name Fms-related tyrosine kinase 3 ligand (Flt3 ligand), Human, ELISA assay
- Product Description The Human FLT3LG ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal FLT3LG antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FLT3LG concentration are added to these wells and allowed to complex with the bound n FLT3LG antibody. A biotinylated FLT3LG goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FLT3LG present in the samples. The purpose of this kit is the in-vitro quantitative determination of Human FLT3LG in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Fms-related tyrosine kinase 3 ligand; Flt3 Ligand; Flt3L;
- Application(s) ELISA
- Specificity Human Flt3
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human Flt3 Ligand expression system sf21; Immunogen sequence: T27-P185
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Human FLT3LG ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal FLT3LG antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FLT3LG concentration are added to these wells and allowed to complex with the bound n FLT3LG antibody. A biotinylated FLT3LG goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FLT3LG present in the samples. The purpose of this kit is the in-vitro quantitative determination of Human FLT3LG in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Fms-related tyrosine kinase 3 ligand (Flt3 ligand) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Fms-related tyrosine kinase 3 ligand (Flt3 ligand)
- Specificity Human Flt3
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human Flt3 Ligand expression system sf21; Immunogen sequence: T27-P185
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), human Flt3 Ligand protein standards, human Flt3 Ligand biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Fms-related tyrosine kinase 3 ligand; Flt3 Ligand; Flt3L;
- Uniprot Number P49771
- Uniprot Number/Name P49771 (FLT3L_HUMAN)
- Scientific Background Fms-related tyrosine kinase 3 ligand (FLT3LG), is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors (Uniprot).
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
