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Granulocyte colony-stimulating factor (G-CSF), Mouse, ELISA assay
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Catalog Number
BEK-2021
- Product Name Granulocyte colony-stimulating factor (G-CSF), Mouse, ELISA assay
- Product Description The mouse GM-CSF Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse GM-CSF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known GM-CSF concentration are added to these wells and allowed to complex with the bound GM-CSF antibody. A biotinylated mouse GM-CSF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of GM-CSF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse GM-CSF in samples such as culture supernatant, cell lysates, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names GM-CSF; Colony-stimulating factor; Csf2; Csfgm;
- Application(s) ELISA
- Specificity Mouse GM-CSF
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A18-K141
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse GM-CSF Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse GM-CSF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known GM-CSF concentration are added to these wells and allowed to complex with the bound GM-CSF antibody. A biotinylated mouse GM-CSF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of GM-CSF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse GM-CSF in samples such as culture supernatant, cell lysates, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Granulocyte colony-stimulating factor (G-CSF) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Granulocyte colony-stimulating factor (G-CSF)
- Specificity Mouse GM-CSF
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A18-K141
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names GM-CSF; Colony-stimulating factor; Csf2; Csfgm;
- Uniprot Number P01587
- Uniprot Number/Name P01587 (CSF2_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Austin SA, Santhanam AV, Hinton DJ, Choi DS, Katusic ZS. (2013) Endothelial nitric oxide deficiency promotes Alzheimer disease pathology. J Neurochem. 2013 Dec;127(5):691-700.