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Hepatocyte growth factor (HGF), Human, ELISA assay
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US$467.00
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Catalog Number
BEK-2022
- Product Name Hepatocyte growth factor (HGF), Human, ELISA assay
-
Product Description
For quantitative detection of human HGF in cell culture supernatants, serum, and citrate, or EDTA treated plasma samples only when used as directed
The human Hepatocyte Growth Factor (HGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human HGF antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known HGF concentration are added to these wells and allowed to complex with the bound HGF antibody. A biotinylated anti-human HGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of HGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human HGF in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Range: 125 pg/mL - 8,000 pg/mL; Sensitivity <5 pg/mL - Alternative Names Scatter factor; Hepatopoeitin-A; SF; HGF;
- Application(s) ELISA
- Specificity Human HGF
- Species Reactivity Human
- Immunogen Description Standard is recombinant HGF alpha and beta protein produced in and purified from sf21 insect cell line systems. Immunogen sequence: amino acids 32-494 for alpha chain, and amino acids 495-728 for the beta chain of HGF
- Range 125 pg/mL - 8,000 pg/mL
- Sensitivity < 5 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
For quantitative detection of human HGF in cell culture supernatants, serum, and citrate, or EDTA treated plasma samples only when used as directed
The human Hepatocyte Growth Factor (HGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human HGF antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known HGF concentration are added to these wells and allowed to complex with the bound HGF antibody. A biotinylated anti-human HGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of HGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human HGF in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Range: 125 pg/mL - 8,000 pg/mL; Sensitivity <5 pg/mL - Application(s) ELISA
- Application Details ELISA. For the quantification of Hepatocyte growth factor (HGF) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Hepatocyte growth factor (HGF)
- Specificity Human HGF
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Standard is recombinant HGF alpha and beta protein produced in and purified from sf21 insect cell line systems. Immunogen sequence: amino acids 32-494 for alpha chain, and amino acids 495-728 for the beta chain of HGF
- Range 125 pg/mL - 8,000 pg/mL
- Sensitivity < 5 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Scatter factor; Hepatopoeitin-A; SF; HGF;
- Uniprot Number P14210
- Uniprot Number/Name P14210 (HGF_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
