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Inhibin beta A chain, Human, ELISA assay
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Catalog Number
BEK-2001
- Product Name Inhibin beta A chain, Human, ELISA assay
- Product Description The human Activin A Kit is a sandwich ELISA. The capture antibody is a monoclonal Activin A antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Activin A concentration are added to these wells and allowed to complex with the bound Activin A antibody. A biotinylated Activin A monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Activin A present in the samples. The purpose of this kit is the in-vitro quantitative determination of Activin A in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and saliva.
- Alternative Names Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein; EDF; INHBA;Activin A;
- Application(s) ELISA
- Specificity Human Activin A
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids G311-S426
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 12 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Activin A Kit is a sandwich ELISA. The capture antibody is a monoclonal Activin A antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Activin A concentration are added to these wells and allowed to complex with the bound Activin A antibody. A biotinylated Activin A monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Activin A present in the samples. The purpose of this kit is the in-vitro quantitative determination of Activin A in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and saliva.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Inhibin beta A chain in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), Saliva. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Inhibin beta A chain
- Specificity Human Activin A
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids G311-S426
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 12 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Saliva, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein; EDF; INHBA;Activin A;
- Uniprot Number P08476
- Uniprot Number/Name P08476 (INHBA_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
