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Insulin-like growth factor 1 receptor (IGF1R), Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2346
- Product Name Insulin-like growth factor 1 receptor (IGF1R), Human, ELISA assay
- Product Description The Human IGF-1 receptor kit is a sandwich ELISA. The capture antibody is a mouse monoclonal IGF-1 receptor antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IGF-1 receptor concentration are added to these wells and allowed to complex with the bound IGF-1 receptor antibody. A biotinylated IGF-1 receptor goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGF-1 receptor present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IGF-1 receptor in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Insulin-like growth factor 1 receptor; IGF-1 receptor; CD221;IGF1R;
- Application(s) ELISA
- Specificity Human IGF-1 receptor protein
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant Human IGF1R expressed in a NSO expression system; Immunogen sequence: E31-N932
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Human IGF-1 receptor kit is a sandwich ELISA. The capture antibody is a mouse monoclonal IGF-1 receptor antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IGF-1 receptor concentration are added to these wells and allowed to complex with the bound IGF-1 receptor antibody. A biotinylated IGF-1 receptor goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGF-1 receptor present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IGF-1 receptor in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Insulin-like growth factor 1 receptor (IGF1R) in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Insulin-like growth factor 1 receptor (IGF1R)
- Specificity Human IGF-1 receptor protein
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant Human IGF1R expressed in a NSO expression system; Immunogen sequence: E31-N932
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), Human IGF-1 receptor protein standards, Human IGF-1 receptor biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Insulin-like growth factor 1 receptor; IGF-1 receptor; CD221;IGF1R;
- Uniprot Number P08069
- Uniprot Number/Name P08069 (IGF1R_HUMAN)
- Scientific Background IGF-1 receptor binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF-1R is involved in cell growth and survival control. IGF-1 receptor is crucial for tumor transformation and survival of malignant cells.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
