Intercellular adhesion molecule 1, soluble (sICAM-1), Human, ELISA assay

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Catalog Number
BEK-2023

    Product Info

  • Product Name Intercellular adhesion molecule 1, soluble (sICAM-1), Human, ELISA assay
  • Product Description google The human ICAM-1 Kit is a sandwich ELISA. The capture antibody is a monoclonal human ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated human ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human ICAM-1 in samples such as culture supernatant, serum, cell lysates, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54; soluble ICAM-1
  • Application(s) ELISA
  • Specificity Human ICAM-1
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q28-E480
  • Range 156 pg/mL - 10,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human ICAM-1 Kit is a sandwich ELISA. The capture antibody is a monoclonal human ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated human ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human ICAM-1 in samples such as culture supernatant, serum, cell lysates, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Intercellular adhesion molecule 1, soluble (sICAM-1) in Culture Supernatant, Serum, Cell Lysates, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Intercellular adhesion molecule 1, soluble (sICAM-1)
  • Specificity Human ICAM-1
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q28-E480
  • Range 156 pg/mL - 10,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54; soluble ICAM-1
  • Uniprot Number P05362
  • Uniprot Number/Name P05362 (ICAM1_HUMAN)
  • Scientific Background Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system.
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References Nonaka, K. et al. (2017) Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-κB pathways in human gingival fibroblasts. J Periodontal Res. [Epub ahead of print] Application: Cell lysate