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Intercellular adhesion molecule 1, soluble (sICAM-1), Rat, ELISA assay
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Catalog Number
BEK-2025
- Product Name Intercellular adhesion molecule 1, soluble (sICAM-1), Rat, ELISA assay
- Product Description The rat ICAM-1 Kit is a sandwich ELISA. The capture antibody is a mouse monoclonal ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated rat ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat ICAM-1/CD54 in culture supernatant, cell lysates, serum, plasma (EDTA) and plasma (Heparin) samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54; Icam1; Icam-1;
- Application(s) ELISA
- Specificity Rat ICAM-1
- Species Reactivity Rat
- Immunogen Description The standard product used in this kit is recombinant rat ICAM-1/CD54, consisting amino acids 28-493 expressed in and purified from a mammalian cell expression system..
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 2 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The rat ICAM-1 Kit is a sandwich ELISA. The capture antibody is a mouse monoclonal ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated rat ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat ICAM-1/CD54 in culture supernatant, cell lysates, serum, plasma (EDTA) and plasma (Heparin) samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Intercellular adhesion molecule 1, soluble (sICAM-1) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Intercellular adhesion molecule 1, soluble (sICAM-1)
- Specificity Rat ICAM-1
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard product used in this kit is recombinant rat ICAM-1/CD54, consisting amino acids 28-493 expressed in and purified from a mammalian cell expression system..
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 2 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54; Icam1; Icam-1;
- Uniprot Number Q00238
- Uniprot Number/Name Q00238 (ICAM1_RAT)
- Scientific Background Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
