Interferon gamma (IFN-gamma), Human, ELISA assay

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Catalog Number
BEK-2026

    Product Info

  • Product Name Interferon gamma (IFN-gamma), Human, ELISA assay
  • Product Description google The human Interferon gamma Kit is a sandwich ELISA. The capture antibody is a monoclonal human Interferon-gamma antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Interferon gamma concentration are added to these wells and allowed to complex with the bound Interferon gamma antibody. A biotinylated human Interferon-gamma specific polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Interferon gamma present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Interferon gamma in culture supernatant and serum only. It is not recommended for additional substrates at this time. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names Immune interferon; IFNG; interferon gamma
  • Application(s) ELISA
  • Specificity Human Interferon gamma
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Q24-Q166
  • Range 15.6 pg/mL - 1,000 pg/mL
  • Sensitivity < 2 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human Interferon gamma Kit is a sandwich ELISA. The capture antibody is a monoclonal human Interferon-gamma antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Interferon gamma concentration are added to these wells and allowed to complex with the bound Interferon gamma antibody. A biotinylated human Interferon-gamma specific polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Interferon gamma present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Interferon gamma in culture supernatant and serum only. It is not recommended for additional substrates at this time. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Interferon gamma (IFN-gamma) in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Interferon gamma (IFN-gamma)
  • Specificity Human Interferon gamma
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Q24-Q166
  • Range 15.6 pg/mL - 1,000 pg/mL
  • Sensitivity < 2 pg/mL
  • Sample Type Culture Supernatant, Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names Immune interferon; IFNG; interferon gamma
  • Uniprot Number P01579
  • Uniprot Number/Name P01579 (IFNG_HUMAN)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References Guo F et al. (2017) Human regulatory macrophages are potent in suppression of the xenoimmune response via indoleamine-2,3-dioxygenase-involved mechanism(s). Xenotransplantation. [Epub ahead of print] Application: Culture supernatant.