Product DescriptiongoogleThe mouse Interleukin 1 alpha (IL-1 alpha) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-1 alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-1 alpha concentration are added to these wells and allowed to complex with the bound IL-1 alpha antibody. A biotinylated mouse IL-1 alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-1 alpha in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesInterleukin-1 alpha; IL-1 alpha; Il1a; IL-1a; Interleukin 1a;
Application(s)ELISA
SpecificityMouse IL-1a
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S115-S270
Product DescriptionThe mouse Interleukin 1 alpha (IL-1 alpha) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-1 alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-1 alpha concentration are added to these wells and allowed to complex with the bound IL-1 alpha antibody. A biotinylated mouse IL-1 alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-1 alpha in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Interleukin-1 alpha (IL-1 alpha) in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetInterleukin-1 alpha (IL-1 alpha)
SpecificityMouse IL-1a
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesMouse
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S115-S270
Range4.7 pg/mL - 300 pg/mL
Sensitivity< 1 pg/mL
Sample TypeCulture Supernatant, Serum
Detection MethodColorimetric
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesInterleukin-1 alpha; IL-1 alpha; Il1a; IL-1a; Interleukin 1a;