Product NameInterleukin-1 alpha (IL-1 alpha), Rat, ELISA assay
Product DescriptiongoogleThe rat Interleukin 1 alpha (IL-1 alpha) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat IL-1 alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IL-1 alpha concentration are added to these wells and allowed to complex with the bound IL-1 alpha antibody. A biotinylated rat IL-1 alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IL-1 alpha in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesInterleukin-1 alpha; IL-1 alpha; Il1a; IL-1a; IL-1;
Application(s)ELISA
SpecificityRat IL-1a
Species ReactivityRat
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S115-S270
Product DescriptionThe rat Interleukin 1 alpha (IL-1 alpha) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat IL-1 alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IL-1 alpha concentration are added to these wells and allowed to complex with the bound IL-1 alpha antibody. A biotinylated rat IL-1 alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IL-1 alpha in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Interleukin-1 alpha (IL-1 alpha) in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetInterleukin-1 alpha (IL-1 alpha)
SpecificityRat IL-1a
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesRat
Species ReactivityRat
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S115-S270
Range4.7 pg/mL - 300 pg/mL
Sensitivity< 0.5 pg/mL
Sample TypeCulture Supernatant, Serum
Detection MethodColorimetric
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesInterleukin-1 alpha; IL-1 alpha; Il1a; IL-1a; IL-1;