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Interleukin-10 (IL-10), Human, ELISA assay
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Catalog Number
BEK-2045
- Product Name Interleukin-10 (IL-10), Human, ELISA assay
- Product Description The human Interleukin 10 (IL-10) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-10 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-10 concentration are added to these wells and allowed to complex with the bound IL-10 antibody. A biotinylated human IL-10 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-10 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-10 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10;
- Application(s) ELISA
- Specificity Human IL-10
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S19-N178
- Range 7.8 pg/mL - 500 pg/mL (cell culture supernates); 3.4 pg/mL - 250 pg/mL (human serum, plasma)
- Sensitivity < 0.5 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Interleukin 10 (IL-10) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-10 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-10 concentration are added to these wells and allowed to complex with the bound IL-10 antibody. A biotinylated human IL-10 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-10 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-10 in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-10 (IL-10) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-10 (IL-10)
- Specificity Human IL-10
- Target Cross-Reactivity (ELISA) There is cross-reactivates with TGFbeta2, TGFbeta3, TGFbeta5<1%.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S19-N178
- Range 7.8 pg/mL - 500 pg/mL (cell culture supernates); 3.4 pg/mL - 250 pg/mL (human serum, plasma)
- Sensitivity < 0.5 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10;
- Uniprot Number P22301
- Uniprot Number/Name P22301 (IL10_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
