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Interleukin-10 (IL-10), Mouse, ELISA assay
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Catalog Number
BEK-2046
- Product Name Interleukin-10 (IL-10), Mouse, ELISA assay
- Product Description The mouse Interleukin 10 (IL-10) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-10 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-10 concentration are added to these wells and allowed to complex with the bound IL-10 antibody. A biotinylated mouse IL-10 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-10 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-10 in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10;
- Application(s) ELISA
- Specificity Natural and recombinant Mouse IL-10
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S19-S178
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Interleukin 10 (IL-10) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-10 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-10 concentration are added to these wells and allowed to complex with the bound IL-10 antibody. A biotinylated mouse IL-10 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-10 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-10 in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-10 (IL-10) in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-10 (IL-10)
- Specificity Natural and recombinant Mouse IL-10
- Target Cross-Reactivity (ELISA) The cross reactivity with TGF_2, TGF_3 and TGF_5 is < 1%.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S19-S178
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Culture Supernatant, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10;
- Uniprot Number P18893
- Uniprot Number/Name P18893 (IL10_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
