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interleukin-12 p70 (IL-12 p70), Mouse, ELISA assay
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Catalog Number
BEK-2049
- Product Name interleukin-12 p70 (IL-12 p70), Mouse, ELISA assay
- Product Description The mouse Interleukin 12 (p70) Kit is a sandwich ELISA. The capture antibody is a monoclonal specific for mouse IL-12(p70) antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and recombinant standards of known IL-12(p70) concentration are added to these wells and allowed to complex with the bound IL-12(p70) antibody. A biotinylated polyclonal IL-12(p70) specific antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-12(p70) present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-12(p70) in samples of culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
- Alternative Names Interleukin-12; IL-12; IL12; IL-12p70
- Application(s) ELISA
- Specificity Mouse IL-12(p70)
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a NSO expression system consisting of amino acids (M23-S335)+(R23-A215)
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1.5 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Interleukin 12 (p70) Kit is a sandwich ELISA. The capture antibody is a monoclonal specific for mouse IL-12(p70) antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and recombinant standards of known IL-12(p70) concentration are added to these wells and allowed to complex with the bound IL-12(p70) antibody. A biotinylated polyclonal IL-12(p70) specific antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-12(p70) present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-12(p70) in samples of culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
- Application(s) ELISA
- Application Details ELISA. For the quantification of interleukin-12 p70 (IL-12 p70) in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target interleukin-12 p70 (IL-12 p70)
- Specificity Mouse IL-12(p70)
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a NSO expression system consisting of amino acids (M23-S335)+(R23-A215)
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1.5 pg/mL
- Sample Type Culture Supernatant, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-12; IL-12; IL12; IL-12p70
- Uniprot Number F8WI71
- Uniprot Number/Name F8WI71 (F8WI71_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
