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Interleukin-17A (IL-17A), Mouse, ELISA assay
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Catalog Number
BEK-2054
- Product Name Interleukin-17A (IL-17A), Mouse, ELISA assay
- Product Description The mouse Interleukin 17 (IL-17) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-17 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-17 concentration are added to these wells and allowed to complex with the bound IL-17 antibody. A biotinylated mouse IL-17 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-17 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-17A in culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA) samples only when used as directed. For research use only, not for clinical or diagnostic use.
- Alternative Names Interleukin-17; IL-17; IL17; Interleukin-17A; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; Il17a; Ctla8; Il17;
- Application(s) ELISA
- Specificity Mouse IL-17A/IL-17
- Species Reactivity Mouse
- Immunogen Description The standard product used in this kit is recombinant mouse IL-17A, consisting amino acids 22-158 expressed in and purified from an E. coli expression system.
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Interleukin 17 (IL-17) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-17 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-17 concentration are added to these wells and allowed to complex with the bound IL-17 antibody. A biotinylated mouse IL-17 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-17 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-17A in culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA) samples only when used as directed. For research use only, not for clinical or diagnostic use.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-17A (IL-17A) in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-17A (IL-17A)
- Specificity Mouse IL-17A/IL-17
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard product used in this kit is recombinant mouse IL-17A, consisting amino acids 22-158 expressed in and purified from an E. coli expression system.
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Interleukin-17; IL-17; IL17; Interleukin-17A; IL-17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8; Il17a; Ctla8; Il17;
- Uniprot Number Q62386
- Uniprot Number/Name Q62386 (IL17_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
- Specific References Chao X et al. (2020) Long-term PM2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis. Aging (Albany NY). 12(12):11579-11602 Application: Mouse, serum.