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Interleukin-6 (IL-6), Human, ELISA assay
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Catalog Number
BEK-2042
- Product Name Interleukin-6 (IL-6), Human, ELISA assay
- Product Description The human Interleukin 6 (IL-6) kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-6 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-6 concentration are added to these wells and allowed to complex with the bound IL-6 antibody. A biotinylated human IL-6 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-6 present in the samples. The purpose of this kit is for quantitative detection of human IL-6 in culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate) samples only when used as directed This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names IL-6; Interleukin-6; B-cell stimulatory factor 2; BSF-2; Interferon beta-2; Hybridoma growth factor; CTL diffenrentiation factor; CDF; IL6; IFNB2;
- Application(s) ELISA
- Specificity Human IL-6
- Species Reactivity Human
- Immunogen Description The standard product used in this kit is recombinant human IL-6, consisting amino acids 29-212 expressed in and purified from an E. coli expression system
- Range 4.7 pg/mL - 300 pg/mL
- Sensitivity < 0.3 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Interleukin 6 (IL-6) kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-6 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-6 concentration are added to these wells and allowed to complex with the bound IL-6 antibody. A biotinylated human IL-6 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-6 present in the samples. The purpose of this kit is for quantitative detection of human IL-6 in culture supernatant, serum, plasma (Heparin), plasma (EDTA) and plasma (Citrate) samples only when used as directed This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Interleukin-6 (IL-6) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Interleukin-6 (IL-6)
- Specificity Human IL-6
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard product used in this kit is recombinant human IL-6, consisting amino acids 29-212 expressed in and purified from an E. coli expression system
- Range 4.7 pg/mL - 300 pg/mL
- Sensitivity < 0.3 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names IL-6; Interleukin-6; B-cell stimulatory factor 2; BSF-2; Interferon beta-2; Hybridoma growth factor; CTL diffenrentiation factor; CDF; IL6; IFNB2;
- Uniprot Number P05231
- Uniprot Number/Name P05231 (IL6_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Yu HC et al. (2021) Brain-Derived Neurotrophic Factor Suppressed Proinflammatory Cytokines Secretion and Enhanced MicroRNA (miR)-3168 Expression in Macrophages. Int J Mol Sci. 23(1):570 Application: Human, cell culture supernatant.
Wickstrom K et al. (2016) Effect of Lignocaine on IL-6, IL-8, and MCP-1 in Peritoneal Macrophages and Endometriotic Stromal Cells. Reprod Sci. [Epub ahead of print] Application: Human, cell culture supernatant.