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Laminin, Mouse, ELISA assay
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Catalog Number
BEK-2056
- Product Name Laminin, Mouse, ELISA assay
- Product Description The mouse Laminin Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Laminin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Laminin concentration are added to these wells and allowed to complex with the bound Laminin antibody. A biotinylated mouse Laminin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Laminin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Laminin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names LAMA; LAMA1;
- Application(s) ELISA
- Specificity Mouse Laminin
- Species Reactivity Mouse
- Immunogen Description Purified mouse Laminin from murine sarcoma basement membrane
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Laminin Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Laminin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Laminin concentration are added to these wells and allowed to complex with the bound Laminin antibody. A biotinylated mouse Laminin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Laminin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Laminin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Laminin in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Laminin
- Specificity Mouse Laminin
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description Purified mouse Laminin from murine sarcoma basement membrane
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names LAMA; LAMA1;
- Uniprot Number P19137
- Uniprot Number/Name P19137 (LAMA1_MOUSE)
- Scientific Background Laminin is a large (900 kDa) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma which are bound to each other by disulphide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
