Product DescriptiongoogleThe Biosensis NGFR/p75ECDRapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a goat anti- p75ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards.
This NGFR/p75ECD ELISA kit employs a recombinant mouse p75ECD-Fc chimera protein as standard. While there is a current lack of a true mouse p75ECD standard, this ELISA kit allows quantification of mouse p75ECD as p75ECD-Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75ECD.
This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product DescriptionThe Biosensis NGFR/p75ECDRapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a goat anti- p75ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards.
This NGFR/p75ECD ELISA kit employs a recombinant mouse p75ECD-Fc chimera protein as standard. While there is a current lack of a true mouse p75ECD standard, this ELISA kit allows quantification of mouse p75ECD as p75ECD-Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75ECD.
This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application DetailsELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Culture Supernatant, Urine, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Scientific BackgroundThe nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Shipping StatementFor order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
Specific ReferencesLuu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse plasma.
Zabbarova IV et al. (2018) Targeting p75 neurotrophin receptors ameliorates spinal cord injury-induced detrusor sphincter dyssynergia in mice. Neurourol Urodyn. 2018 May 28 [Epub ahead of print]. Application: Mouse urine.
Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
General ReferencesDiStefano & Johnson (1988) Proc Natl Acad Sci USA 85: 270-274. Shepheard et al. (2014) PLoS ONE 9(1): e87398. doi:10.1371/journal.pone.0087398.