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Neutrophil gelatinase-associated lipocalin-2 (NGAL), Rat, ELISA assay
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Catalog Number
BEK-2128
- Product Name Neutrophil gelatinase-associated lipocalin-2 (NGAL), Rat, ELISA assay
- Product Description The rat Lipocalin-2/NGAL Kit is a sandwich ELISA. The capture antibody is a polyclonal rat Lipocalin-2/NGAL antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Lipocalin-2/NGAL concentration are added to these wells and allowed to complex with the bound Lipocalin-2/NGAL antibody. A biotinylated rat Lipocalin-2/NGAL polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2/NGAL present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Lipocalin-2/NGAL in samples such as culture supernatant, serum, plasma (Heparin) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Neutrophil gelatinase-associated lipocalin; NGAL; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; p25; Lcn2;
- Application(s) ELISA
- Specificity Rat Lipocalin-2/NGAL
- Species Reactivity Rat
- Immunogen Description The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant rat Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system.
- Range 78 pg/mL - 5000 pg/mL
- Sensitivity < 15 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The rat Lipocalin-2/NGAL Kit is a sandwich ELISA. The capture antibody is a polyclonal rat Lipocalin-2/NGAL antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Lipocalin-2/NGAL concentration are added to these wells and allowed to complex with the bound Lipocalin-2/NGAL antibody. A biotinylated rat Lipocalin-2/NGAL polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2/NGAL present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Lipocalin-2/NGAL in samples such as culture supernatant, serum, plasma (Heparin) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Neutrophil gelatinase-associated lipocalin-2 (NGAL) in Culture Supernatant, Serum, Plasma (Heparin), Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Neutrophil gelatinase-associated lipocalin-2 (NGAL)
- Specificity Rat Lipocalin-2/NGAL
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant rat Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system.
- Range 78 pg/mL - 5000 pg/mL
- Sensitivity < 15 pg/mL
- Sample Type Culture Supernatant, Plasma (Heparin), Serum, Urine
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Neutrophil gelatinase-associated lipocalin; NGAL; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; p25; Lcn2;
- Uniprot Number P30152
- Uniprot Number/Name P30152 (NGAL_RAT)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
