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Osteopontin, Human, ELISA assay
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Catalog Number
BEK-2083
- Product Name Osteopontin, Human, ELISA assay
- Product Description The human Osteopontin (OPN) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human OPN antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known OPN concentration are added to these wells and allowed to complex with the bound OPN antibody. A biotinylated human OPN polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPN present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OPN in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA), human milk and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP; OPN;
- Application(s) ELISA
- Specificity Human OPN
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids I17-N300
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Osteopontin (OPN) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human OPN antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known OPN concentration are added to these wells and allowed to complex with the bound OPN antibody. A biotinylated human OPN polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPN present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OPN in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA), human milk and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Osteopontin in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Human Milk, Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Osteopontin
- Specificity Human OPN
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids I17-N300
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Human Milk, Plasma (EDTA), Plasma (Heparin), Serum, Urine
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP; OPN;
- Uniprot Number P10451
- Uniprot Number/Name P10451 (OSTP_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Karamizadeh Z et al. (2013) Investigation of osteopontin levels and genomic variation of osteopontin and its receptors in Type 1 diabetes mellitus. J Endocrinol Invest. 2013 Dec;36(11):1090-3.