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P-selectin (sP-selectin), Mouse, ELISA assay
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Catalog Number
BEK-2091
- Product Name P-selectin (sP-selectin), Mouse, ELISA assay
- Product Description The mouse P-Selectin Kit is a sandwich ELISA. The mouse capture antibody is an anti-mouse P-Selectin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known P-Selectin concentration are added to these wells and allowed to complex with the bound P-Selectin antibody. A biotinylated anti-mouse P-Selectin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of P-Selectin present in the samples. The purpose of this kit is the in-vitro quantitative detection of mouse P-Selectin in culture supernatant, serum and plasma (Citrate) samples only when used as directed. This assay has not been tested on tissue lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P; SELP; GMRP; GRMP; sP-selectin;
- Application(s) ELISA
- Specificity Mouse P-Selectin, soluble P-Selectin, s(P-Selectin)
- Species Reactivity Mouse
- Immunogen Description Standard is recombinant mouse P-selectin, amino acids 42-709 expressed in and purified from a mammalian cell expression system. Amino acids 42-709 represent the ECD domain of mouse P-selectin, so this ELISA will detect primarily soluble P-selectin in its targeted substrates but fully length P-selectin if available could compete as well.
- Range 312 pg/mL - 20,000 pg/mL
- Sensitivity < 5 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse P-Selectin Kit is a sandwich ELISA. The mouse capture antibody is an anti-mouse P-Selectin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known P-Selectin concentration are added to these wells and allowed to complex with the bound P-Selectin antibody. A biotinylated anti-mouse P-Selectin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of P-Selectin present in the samples. The purpose of this kit is the in-vitro quantitative detection of mouse P-Selectin in culture supernatant, serum and plasma (Citrate) samples only when used as directed. This assay has not been tested on tissue lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of P-selectin (sP-selectin) in Culture Supernatant, Serum, Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target P-selectin (sP-selectin)
- Specificity Mouse P-Selectin, soluble P-Selectin, s(P-Selectin)
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description Standard is recombinant mouse P-selectin, amino acids 42-709 expressed in and purified from a mammalian cell expression system. Amino acids 42-709 represent the ECD domain of mouse P-selectin, so this ELISA will detect primarily soluble P-selectin in its targeted substrates but fully length P-selectin if available could compete as well.
- Range 312 pg/mL - 20,000 pg/mL
- Sensitivity < 5 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P; SELP; GMRP; GRMP; sP-selectin;
- Uniprot Number Q01102
- Uniprot Number/Name Q01102 (LYAM3_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
