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Persephin, Human, ELISA assay
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US$757.00
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Catalog Number
BEK-2352
- Product Name Persephin, Human, ELISA assay
- Product Description The human Persephin kit is a sandwich ELISA. The capture antibody is a human Persephin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Persephin concentration are added to these wells and allowed to complex with the bound human Persephin antibody. A biotinylated human Persephin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human Persephin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Persephin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names PSP; PSPN;
- Application(s) ELISA
- Specificity Human Persephin protein
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human Persephin expressed in an E.coli expression system; Immunogen sequence: W22-G156
- Range 93.7 pg/mL - 6,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Persephin kit is a sandwich ELISA. The capture antibody is a human Persephin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Persephin concentration are added to these wells and allowed to complex with the bound human Persephin antibody. A biotinylated human Persephin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human Persephin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Persephin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Persephin in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Persephin
- Specificity Human Persephin protein
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human Persephin expressed in an E.coli expression system; Immunogen sequence: W22-G156
- Range 93.7 pg/mL - 6,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), human Persephin protein standards, human Persephin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names PSP; PSPN;
- Uniprot Number O60542
- Uniprot Number/Name O60542 (PSPN_HUMAN)
- Scientific Background Persephin exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
