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Resistin, Human, ELISA assay
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Catalog Number
BEK-2115
- Product Name Resistin, Human, ELISA assay
- Product Description The human Resistin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Resistin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Resistin concentration are added to these wells and allowed to complex with the bound Resistin antibody. A biotinylated human Resistin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Resistin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Resistin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; RETN; FIZZ3; HXCP1; RSTN;
- Application(s) ELISA
- Specificity Human Resistin
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids P2-A115
- Range 78 pg/mL - 5000 pg/mL
- Sensitivity < 3 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Resistin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Resistin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Resistin concentration are added to these wells and allowed to complex with the bound Resistin antibody. A biotinylated human Resistin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Resistin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Resistin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Resistin in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Resistin
- Specificity Human Resistin
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids P2-A115
- Range 78 pg/mL - 5000 pg/mL
- Sensitivity < 3 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; RETN; FIZZ3; HXCP1; RSTN;
- Uniprot Number Q9HD89
- Uniprot Number/Name Q9HD89 (RETN_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
