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Thrombopoietin (TPO), Mouse, ELISA assay
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Catalog Number
BEK-2096
- Product Name Thrombopoietin (TPO), Mouse, ELISA assay
- Product Description The Biosensis Mouse Thrombopoietin/TPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody specific for Thrombopoietin/TPO has been precoated onto 96-well plates. Standards and test samples are added to the wells, and then a biotinylated detection polyclonal antibody specific for Thrombopoietin/TPO is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates washed away again with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP driven enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that is changed into yellow by the addition of an acid stop solution. The intensity of this coloured product is directly proportional to the concentration of TPO present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TPO in samples such as culture supernatant, tissue homogenates, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names TPO; C-mpl ligand; mL; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; Thpo;
- Application(s) ELISA
- Specificity Mouse TPO
- Species Reactivity Mouse
- Immunogen Description The standard and antigen for this mouse TPO ELISA is recombinant mouse TPO produced in a mammalian cell expression system and purified, aa.22-356 of mouse TPO.
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 5 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Biosensis Mouse Thrombopoietin/TPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody specific for Thrombopoietin/TPO has been precoated onto 96-well plates. Standards and test samples are added to the wells, and then a biotinylated detection polyclonal antibody specific for Thrombopoietin/TPO is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates washed away again with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP driven enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that is changed into yellow by the addition of an acid stop solution. The intensity of this coloured product is directly proportional to the concentration of TPO present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TPO in samples such as culture supernatant, tissue homogenates, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Thrombopoietin (TPO) in Culture Supernatant, Tissue Homogenates, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Thrombopoietin (TPO)
- Specificity Mouse TPO
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and antigen for this mouse TPO ELISA is recombinant mouse TPO produced in a mammalian cell expression system and purified, aa.22-356 of mouse TPO.
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 5 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Tissue Homogenates
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names TPO; C-mpl ligand; mL; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; Thpo;
- Uniprot Number P40226
- Uniprot Number/Name P40226 (TPO_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
