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Transforming growth factor beta-1 proprotein (TGFB1), Human, ELISA assay
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Catalog Number
BEK-2093
- Product Name Transforming growth factor beta-1 proprotein (TGFB1), Human, ELISA assay
- Product Description The human Transforming growth factor beta-1 (TGFb1) Kit is a sandwich ELISA. The capture antibody is a monoclonal TGFb1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TGFb1 concentration are added to these wells and allowed to complex with the bound TGFb1 antibody. A biotinylated TGFb1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TGFb1 present in the samples. There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%. The purpose of this kit is the in-vitro quantitative determination of human TGFb1 in culture supernatant, serum and plasma (EDTA). Pretreatment of samples with acid precipitation and neutralization step is required for optimal activity. Note: TGFbeta 1 is naturally very liable once released from its natural binding complex. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names TGFB1; TGFB; TGF-beta-1;
- Application(s) ELISA
- Specificity TGF Beta 1. The kit will react with Human, Mouse and Rat. Cross reactivity with TGF Beta 2, TGF Beta 3, and TGF Beta 5 is less than 1%.
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A279-S390
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Transforming growth factor beta-1 (TGFb1) Kit is a sandwich ELISA. The capture antibody is a monoclonal TGFb1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TGFb1 concentration are added to these wells and allowed to complex with the bound TGFb1 antibody. A biotinylated TGFb1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TGFb1 present in the samples. There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%. The purpose of this kit is the in-vitro quantitative determination of human TGFb1 in culture supernatant, serum and plasma (EDTA). Pretreatment of samples with acid precipitation and neutralization step is required for optimal activity. Note: TGFbeta 1 is naturally very liable once released from its natural binding complex. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Transforming growth factor beta-1 proprotein (TGFB1) in Culture Supernatant, Serum, Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Transforming growth factor beta-1 proprotein (TGFB1)
- Specificity TGF Beta 1. The kit will react with Human, Mouse and Rat. Cross reactivity with TGF Beta 2, TGF Beta 3, and TGF Beta 5 is less than 1%.
- Target Cross-Reactivity (ELISA) Cross reactivity with TGF Beta 2, TGF Beta 3, and TGF Beta 5 is less than 1%.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A279-S390
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names TGFB1; TGFB; TGF-beta-1;
- Uniprot Number P01137
- Uniprot Number/Name P01137 (TGFB1_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Pan S et al. (2022) TGF-β1 is associated with deficits in cognition and cerebral cortical thickness in first-episode schizophrenia. J Psychiatry Neurosci. 47(2):E86-E98 Application: Human, plasma.
Moharami S et al. (2021) Investigation of serum levels of orexin-A, transforming growth factor β, and leptin in patients with multiple sclerosis. J Clin Lab Anal. [Epub ahead of print] Application: Human, serum.