Product DescriptiongoogleThe mouse Transforming growth factor beta-1 (TGFb1) Kit is a sandwich ELISA. The capture antibody is a monoclonal TGFb1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TGFb1 concentration are added to these wells and allowed to complex with the bound TGFb1 antibody. A biotinylated TGFb1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TGFb1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TGFb1 in culture supernatant, serum, plasma (EDTA) and urine. Acid pretreatment is required for best activity. Free TGFB1 is high labile. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesTGFB1; TGFB; TGF-beta-1;
Application(s)ELISA
SpecificityHuman; Mouse; Rat; The cross reactivity with TGFbeta2, TGFbeta3 and TGFbeta5 is < 1%.
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A279-S390
Product DescriptionThe mouse Transforming growth factor beta-1 (TGFb1) Kit is a sandwich ELISA. The capture antibody is a monoclonal TGFb1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TGFb1 concentration are added to these wells and allowed to complex with the bound TGFb1 antibody. A biotinylated TGFb1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TGFb1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TGFb1 in culture supernatant, serum, plasma (EDTA) and urine. Acid pretreatment is required for best activity. Free TGFB1 is high labile. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Transforming growth factor beta-1 proprotein (TGFB1) in Culture Supernatant, Serum, Plasma (EDTA), Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
SpecificityHuman; Mouse; Rat; The cross reactivity with TGFbeta2, TGFbeta3 and TGFbeta5 is < 1%.
Target Cross-Reactivity (ELISA)The cross reactivity with TGF Beta 2, TGF Beta 3 and TGF Beta 5 is < 1%.
Target Host SpeciesMouse
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A279-S390
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specific ReferencesFeng F et al. (2021) TIPE2 Suppresses Malignancy of Pancreatic Cancer Through Inhibiting TGFβ1 Mediated Signaling Pathway. Front Oncol. 11:680985. Application: Mouse cell culture supernatant.
Elaskalani O et al. (2020) Antiplatelet Drug Ticagrelor Enhances Chemotherapeutic Efficacy by Targeting the Novel P2Y12-AKT Pathway in Pancreatic Cancer Cells. Cancers (Basel). 12(1). Application: Mouse serum.