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Tumor necrosis factor alpha (TNF-alpha), Human, ELISA assay
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Catalog Number
BEK-2100
- Product Name Tumor necrosis factor alpha (TNF-alpha), Human, ELISA assay
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Product Description
Tumor necrosis factor alpha (TNF-alpha) is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF-alpha is mainly secreted by macrophages. TNF-alpha is involved in the regulation of many processes such as cell proliferation, differentiation and apoptosis and has been implicated in a variety of diseases including cancer. TNF-alpha is expressed as a single-pass type II membrane protein. A soluble, secreted form is derived from the membrane form by proteolytic processing.
The human TNF-alpha Kit is a sandwich ELISA. The capture antibody is a monoclonal human TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated human TNF-alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-alpha in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. - Alternative Names TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
- Application(s) ELISA
- Specificity Human TNF-alpha
- Species Reactivity Human
- Immunogen Description The standard used in this human TNF alpha kit was also used as the antigen for the antibodies. It is a recombinant human protein fragment expressed in e. coli and purified. The exact sequence maps to the soluble form of TNF alpha, amino acids 77-233.
- Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (serum, plasma)
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
Tumor necrosis factor alpha (TNF-alpha) is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF-alpha is mainly secreted by macrophages. TNF-alpha is involved in the regulation of many processes such as cell proliferation, differentiation and apoptosis and has been implicated in a variety of diseases including cancer. TNF-alpha is expressed as a single-pass type II membrane protein. A soluble, secreted form is derived from the membrane form by proteolytic processing.
The human TNF-alpha Kit is a sandwich ELISA. The capture antibody is a monoclonal human TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated human TNF-alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-alpha in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. - Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor necrosis factor alpha (TNF-alpha) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor necrosis factor alpha (TNF-alpha)
- Specificity Human TNF-alpha
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard used in this human TNF alpha kit was also used as the antigen for the antibodies. It is a recombinant human protein fragment expressed in e. coli and purified. The exact sequence maps to the soluble form of TNF alpha, amino acids 77-233.
- Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (serum, plasma)
- Sensitivity < 1 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
- Uniprot Number P01375
- Uniprot Number/Name P01375 (TNFA_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
- Specific References Yu HC et al. (2021) Brain-Derived Neurotrophic Factor Suppressed Proinflammatory Cytokines Secretion and Enhanced MicroRNA (miR)-3168 Expression in Macrophages. Int J Mol Sci. 23(1):570 Application: Human, cell culture supernatant.