Product DescriptiongoogleThe mouse Osteoprotegerin (OPG) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse OPG antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known OPG concentration are added to these wells and allowed to complex with the bound OPG antibody. A biotinylated mouse OPG polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPG present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse OPG in samples such as culture supernatant, serum and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesOsteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; Osteoprotegerin;
Application(s)ELISA
SpecificityMouse OPG
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E22-L401
Product DescriptionThe mouse Osteoprotegerin (OPG) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse OPG antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known OPG concentration are added to these wells and allowed to complex with the bound OPG antibody. A biotinylated mouse OPG polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPG present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse OPG in samples such as culture supernatant, serum and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Tumor necrosis factor receptor superfamily member 11B (Osteoprotegerin) in Culture Supernatant, Serum, Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetTumor necrosis factor receptor superfamily member 11B (Osteoprotegerin)
SpecificityMouse OPG
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesMouse
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E22-L401
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesOsteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; Osteoprotegerin;