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Tumor necrosis factor receptor superfamily member 6 (soluble FAS), Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2014
- Product Name Tumor necrosis factor receptor superfamily member 6 (soluble FAS), Human, ELISA assay
- Product Description The human soluble FAS Kit is a sandwich ELISA. The capture antibody is a monoclonal human sFAS antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known sFAS concentration are added to these wells and allowed to complex with the bound sFAS antibody. A biotinylated human sFAS polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sFAS present in the samples. The purpose of this kit is the in-vitro quantitative determination of human sFAS in samples such as culture supernatant, serum, plasma (EDTA), plasma (Citrate) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names FASLG receptor; Apo-1; CD95; Apt1; Tnfrsf6; FAS; soluble FAS;
- Application(s) ELISA
- Specificity Human sFAS
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids R17-N173
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 3 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human soluble FAS Kit is a sandwich ELISA. The capture antibody is a monoclonal human sFAS antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known sFAS concentration are added to these wells and allowed to complex with the bound sFAS antibody. A biotinylated human sFAS polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sFAS present in the samples. The purpose of this kit is the in-vitro quantitative determination of human sFAS in samples such as culture supernatant, serum, plasma (EDTA), plasma (Citrate) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor necrosis factor receptor superfamily member 6 (soluble FAS) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Citrate), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor necrosis factor receptor superfamily member 6 (soluble FAS)
- Specificity Human sFAS
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids R17-N173
- Range 31.2 pg/mL - 2,000 pg/mL
- Sensitivity < 3 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names FASLG receptor; Apo-1; CD95; Apt1; Tnfrsf6; FAS; soluble FAS;
- Uniprot Number P25445
- Uniprot Number/Name P25445 (TNR6_HUMAN)
- Scientific Background sFAS is a single-pass type I membrane protein and a member of the TNF-receptor superfamily. The interaction between this receptor and the sFAS ligand (TNFSF6/FASLG) forms a death-inducing signalling complex that includes Fas-associated death domain protein (FADD), caspase 8 and caspase 10. Processing of the caspases in this complex leads to apoptosis.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
