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Tyrosine Kinase Receptor A (TrkA), Rat, ELISA assay
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US$547.00
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Catalog Number
BEK-2201
- Product Name Tyrosine Kinase Receptor A (TrkA), Rat, ELISA assay
- Product Description The rat TrkA Kit is a sandwich ELISA. The capture antibody is a polyclonal rat TrkA antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known TrkA concentration are added to these wells and allowed to complex with the bound TrkA antibody. A biotinylated rat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkA present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TrkA in samples such as culture supernatant and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK; TrkA;
- Application(s) ELISA
- Specificity Rat TrkA
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A33-P418
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The rat TrkA Kit is a sandwich ELISA. The capture antibody is a polyclonal rat TrkA antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known TrkA concentration are added to these wells and allowed to complex with the bound TrkA antibody. A biotinylated rat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkA present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TrkA in samples such as culture supernatant and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tyrosine Kinase Receptor A (TrkA) in Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tyrosine Kinase Receptor A (TrkA)
- Specificity Rat TrkA
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A33-P418
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK; TrkA;
- Uniprot Number P35739
- Uniprot Number/Name P35739 (NTRK1_RAT)
- Scientific Background TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
