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Vascular endothelial growth factor A (VEGF-A), Rat, ELISA assay
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Catalog Number
BEK-2109
- Product Name Vascular endothelial growth factor A (VEGF-A), Rat, ELISA assay
- Product Description The rat Vascular endothelial growth factor (VEGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat VEGF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known VEGF concentration are added to these wells and allowed to complex with the bound VEGF antibody. A biotinylated rat VEGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat VEGF in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF;
- Application(s) ELISA
- Specificity Rat VEGF
- Species Reactivity Rat
- Immunogen Description The recombinant immunogen and standard used in this ELISA was manufactured in a mouse myeloma (NSO) expression system, and consisted of amino acids 27-190 rat VEGF-A.
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The rat Vascular endothelial growth factor (VEGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat VEGF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known VEGF concentration are added to these wells and allowed to complex with the bound VEGF antibody. A biotinylated rat VEGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat VEGF in samples such as culture supernatant, serum, plasma (Citrate), plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Vascular endothelial growth factor A (VEGF-A) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Vascular endothelial growth factor A (VEGF-A)
- Specificity Rat VEGF
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The recombinant immunogen and standard used in this ELISA was manufactured in a mouse myeloma (NSO) expression system, and consisted of amino acids 27-190 rat VEGF-A.
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF;
- Uniprot Number P16612
- Uniprot Number/Name P16612 (VEGFA_RAT)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Székiová E et al. (2023) Characterisation of mesenchymal stem cells conditioned media obtained at different conditioning times: their effect on glial cells in in vitro scratch model Growth Factors. [Epub ahead of print] Application: Rat cell culture supernatant.
Sharma D et al. (2021) Local delivery of hydrogel encapsulated vascular endothelial growth factor for the prevention of medication-related osteonecrosis of the jaw. Sci Rep. 11(1):23371 Application: Rat, in-vitro release study.