Product DescriptiongoogleBL-002-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents.
One vial of BL-002-1250 contains 1250 μg IgG which is sufficient as sample diluent additive for one 96-well plate if used at 50 μg/mL. Optimal blocker concentration needs to be validated by the end-user for accurate results.
Product DescriptionBL-002-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents.
One vial of BL-002-1250 contains 1250 μg IgG which is sufficient as sample diluent additive for one 96-well plate if used at 50 μg/mL. Optimal blocker concentration needs to be validated by the end-user for accurate results.
Application(s)ELISA
Application DetailsImmunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays utilizing mouse antibodies.
TargetHeterophilic antibody immunoassay blocker for BEK-2219 and similar ELISA assays
Target Host SpeciesHuman
Species ReactivityHuman
Purity DescriptionPurified
FormatLyophilized.
Reconstitution Instructions1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 1.25 mg/mL. 2) Dilute this stock buffer further with 24 mL assay diluent buffer to obtain the blocker working concentration of 50 µg/mL.
The working concentration of this HA blocker needs to be optimized by the end-user.
Storage InstructionsStore unopened vial at 2-8°C.
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).