Product NameHeterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays
Product DescriptiongoogleBL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.
Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:
BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma BEK-2218, Human NT4/5; Application: Citrate-Plasma
One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.
Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
Product DescriptionBL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.
Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:
BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma BEK-2218, Human NT4/5; Application: Citrate-Plasma
One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.
Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
Application DetailsImmunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
TargetHeterophilic antibody immunoassay blocker for BEK-2226 & BEK-2218 and similar ELISA assays
Target Host SpeciesHuman
Species ReactivityHuman
Purity DescriptionPurified
FormatLyophilized.
Reconstitution Instructions1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 1000 µg/mL. 2) Dilute this stock buffer further with 24 mL assay diluent buffer to obtain the blocker working concentration of 40 µg/mL. Refer to RapidTM ELISA kit protocol for further details.
The working concentration of this HA blocker needs to be optimized by the end-user for other ELISA assays.
Storage InstructionsStore unopened vial at 2-8°C.
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).