Clusterin (Apolipoprotein J, ApoJ), Human, ELISA assay

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Catalog Number
BEK-2304

    Product Info

  • Product Name Clusterin (Apolipoprotein J, ApoJ), Human, ELISA assay
  • Product Description google The human Clusterin (Apolipoprotein J, ApoJ) Kit is a sandwich ELISA. The capture antibody is a monoclonal Clusterin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Clusterin concentration are added to these wells and allowed to complex with the bound Clusterin antibody. A biotinylated Clusterin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Clusterin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Clusterin in samples such as culture supernatant, serum, plasma (heparin, EDTA), saliva and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names Aging-associated gene 4 protein; Apo-J; ApoJ; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Sulfated glycoprotein 2; SGP-2; Testosterone-repressed prostate message 2; TRPM-2; Clusterin beta chain; ApoJalpha; Complement cytolysis inhibitor a chain; Clusterin alpha chain; ApoJbeta; Complement cytolysis inhibitor b chain
  • Application(s) ELISA
  • Specificity This human Clusterin ELISA assay is reactive to clusterin in human samples. There is no detectable cross-reactivity with other relevant proteins.
  • Species Reactivity Human
  • Immunogen Description Expression system for standard: NS0; Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)
  • Range 0.78 ng/mL – 50 ng/mL
  • Sensitivity < 20 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human Clusterin (Apolipoprotein J, ApoJ) Kit is a sandwich ELISA. The capture antibody is a monoclonal Clusterin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Clusterin concentration are added to these wells and allowed to complex with the bound Clusterin antibody. A biotinylated Clusterin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Clusterin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Clusterin in samples such as culture supernatant, serum, plasma (heparin, EDTA), saliva and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Related Products Clusterin (Apolipoprotein J, ApoJ), Rat, ELISA assay
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Clusterin (Apolipoprotein J, ApoJ) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin), saliva, urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Clusterin (Apolipoprotein J, ApoJ)
  • Specificity This human Clusterin ELISA assay is reactive to clusterin in human samples. There is no detectable cross-reactivity with other relevant proteins.
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description Expression system for standard: NS0; Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)
  • Range 0.78 ng/mL – 50 ng/mL
  • Sensitivity < 20 pg/mL
  • Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Saliva, Serum, Urine
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be stored for up to 1 month at 2°C to 8°C.
  • Alternative Names Aging-associated gene 4 protein; Apo-J; ApoJ; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Sulfated glycoprotein 2; SGP-2; Testosterone-repressed prostate message 2; TRPM-2; Clusterin beta chain; ApoJalpha; Complement cytolysis inhibitor a chain; Clusterin alpha chain; ApoJbeta; Complement cytolysis inhibitor b chain
  • Uniprot Number P10909
  • Uniprot Number/Name P10909 CLUS_HUMAN;
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References