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Clusterin (Apolipoprotein J, ApoJ), Mouse, ELISA assay
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US$547.00
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Catalog Number
BEK-2370
- Product Name Clusterin (Apolipoprotein J, ApoJ), Mouse, ELISA assay
- Product Description The mouse Clusterin (Apolipoprotein J, ApoJ). The capture antibody is a monoclonal Clusterin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Clusterin concentration are added to these wells and allowed to complex with the bound Clusterin antibody. A biotinylated Clusterin monoclonal antibody is added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Clusterin present in the samples. The purpose of this kit is the in-vitro quantitaive determination of mouse Clusterin in samples such as cell culture supernatant, cell lysates, serum and plasma (heparin, EDTA). This ELISA kit has not been tested for other applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Clusterin; Apolipoprotein J; Apo-J; Clusterin beta chain; Clusterin alpha chain
- Application(s) ELISA
- Specificity This mouse Clusterin ELISA assay is reactive to clusterin in mouse samples. There is no detectable cross-reactivity with other relevant proteins
- Species Reactivity Mouse
- Immunogen Description Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
- Range 0.31 ng/mL - 20 ng/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse Clusterin (Apolipoprotein J, ApoJ). The capture antibody is a monoclonal Clusterin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Clusterin concentration are added to these wells and allowed to complex with the bound Clusterin antibody. A biotinylated Clusterin monoclonal antibody is added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Clusterin present in the samples. The purpose of this kit is the in-vitro quantitaive determination of mouse Clusterin in samples such as cell culture supernatant, cell lysates, serum and plasma (heparin, EDTA). This ELISA kit has not been tested for other applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Clusterin (Apolipoprotein J, ApoJ) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin) and Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Clusterin (Apolipoprotein J, ApoJ)
- Specificity This mouse Clusterin ELISA assay is reactive to clusterin in mouse samples. There is no detectable cross-reactivity with other relevant proteins
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
- Range 0.31 ng/mL - 20 ng/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Clusterin; Apolipoprotein J; Apo-J; Clusterin beta chain; Clusterin alpha chain
- Uniprot Number Q06890
- Uniprot Number/Name Q06890 Clusterin_MOUSE;
- Scientific Background Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non- caspase-dependent neuronal death following acute brain injury.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
