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Clusterin (Apolipoprotein J, ApoJ), Rat, ELISA assay
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Catalog Number
BEK-2305
- Product Name Clusterin (Apolipoprotein J, ApoJ), Rat, ELISA assay
- Product Description The rat Clusterin (Apolipoprotein J, ApoJ) Kit is a sandwich ELISA. The capture antibody is a monoclonal Clusterin antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Clusterin concentration are added to these wells and allowed to complex with the bound Clusterin antibody. A biotinylated Clusterin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Clusterin present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Clusterin in samples such as culture supernatant, serum and plasma (heparin, EDTA. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2; Clusterin beta chain; Clusterin alpha chain
- Application(s) ELISA
- Specificity This rat Clusterin ELISA assay is reactive to clusterin in rat samples. There is no detectable cross-reactivity with other relevant proteins.
- Species Reactivity Rat
- Immunogen Description Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
- Range 0.31 ng/mL – 20 ng/mL
- Sensitivity < 15 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The rat Clusterin (Apolipoprotein J, ApoJ) Kit is a sandwich ELISA. The capture antibody is a monoclonal Clusterin antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Clusterin concentration are added to these wells and allowed to complex with the bound Clusterin antibody. A biotinylated Clusterin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Clusterin present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Clusterin in samples such as culture supernatant, serum and plasma (heparin, EDTA. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
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Clusterin (Apolipoprotein J, ApoJ), Human, ELISA assay
- Application(s) ELISA
- Application Details ELISA. For the quantification of Clusterin (Apolipoprotein J, ApoJ) in Culture Supernatant, Serum, Plasma (EDTA) and Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Clusterin (Apolipoprotein J, ApoJ)
- Specificity This rat Clusterin ELISA assay is reactive to clusterin in rat samples. There is no detectable cross-reactivity with other relevant proteins.
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description Expression system for standard: NS0; Immunogen sequence: D22-R226 (β) & S227-E448 (α)
- Range 0.31 ng/mL – 20 ng/mL
- Sensitivity < 15 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be stored for up to 1 month at 2°C to 8°C.
- Alternative Names Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2; Clusterin beta chain; Clusterin alpha chain
- Uniprot Number P05371
- Uniprot Number/Name P05371 CLUS_RAT;
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
