Creatinine, urinary, Human, Assay

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Catalog Number
CRE-001

    Product Info

  • Product Name Creatinine, urinary, Human, Assay
  • Product Description google The Biosensis Creatinine (Urinary) Colorimetric Assay can be used to measure creatinine levels in urine. The assay relies on the Jaffe reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate (2). The color derived from creatinine is then destroyed at acidic pH. The difference in color intensity measured at 500 nm before and after acidification is proportional to the creatinine concentration (1,3-4). The sample creatinine concentration is determined using a creatinine standard curve. The Creatinine (Urinary) Colorimetric Assay is not recommended for plasma or serum samples. Assay precision has been determined to be 2.7% (intra-assay, n = 84) and 3.0% (inter-assay, n=5). This assay kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
  • Alternative Names CAS Number 60-27-5;
  • Application(s) Assay
  • Specificity Creatinine (Urinary), kit not recommended for blood
  • Species Reactivity Human, Other Mammals (Predicted)
  • Range 2 - 15 mg/dL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The Biosensis Creatinine (Urinary) Colorimetric Assay can be used to measure creatinine levels in urine. The assay relies on the Jaffe reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate (2). The color derived from creatinine is then destroyed at acidic pH. The difference in color intensity measured at 500 nm before and after acidification is proportional to the creatinine concentration (1,3-4). The sample creatinine concentration is determined using a creatinine standard curve. The Creatinine (Urinary) Colorimetric Assay is not recommended for plasma or serum samples. Assay precision has been determined to be 2.7% (intra-assay, n = 84) and 3.0% (inter-assay, n=5). This assay kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
  • Related Products Nerve growth factor receptor, extracellular domain (NGFR/p75ECD), Human, Rapid™ ELISA assay
  • Application(s) Assay
  • Application Details Assay. For the quantification of Creatinine in Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Creatinine, urinary
  • Specificity Creatinine (Urinary), kit not recommended for blood
  • Target Host Species Human
  • Species Reactivity Human, Other Mammals (Predicted)
  • Range 2 - 15 mg/dL
  • Sample Type Urine
  • Detection Method Colorimetric
  • Kit Components The assay kit box contains 96-well solid microplate(s), creatinine standard, creatinine color reagent, sodium hydroxide, acid solution, sodium borate, surfactant, plate cover and a detailed protocol.
  • Storage Instructions This kit is shipped at room temperature. Upon receipt, store the assay kit at room temperature.
  • Batch Number Please see item label.
  • Expiration Date Refer to expiration date on the outside of the box.
  • Alternative Names CAS Number 60-27-5;
  • Scientific Background Creatine synthesized in kidney, liver, and pancreas is transported in blood to muscle and brain where it is phosphorylated to phosphocreatine. Some free creatine in muscle is converted to creatinine. The amount of creatinine produced is proportional to the individuals muscle mass. In the absence of renal disease, the excretion rate of creatinine in an individual is relatively constant. Thus, urinary creatinine levels may be used as an index of standardization for other tests. Measurement of creatinine clearance is also useful in detecting renal disease and estimating the extent of impairment of renal function (1).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • These standard curves generated in our laboratories are for demonstration purposes only, but can be used as a guide to expected performance. Standard curves should be generated for each assay.

  • Comparison of creatinine values obtained with Biosensis and a competitor kit in normal human urine.

  • Kit Protocol

    CRE-001_as_at_Sep2020.pdf

  • SDS Link

    SDS_CRE-001_as_at_Feb2020.pdf

    Citations & References

  • General References Bowers, L.D. and Wong, E.T. Clin. Chem. 26(5), 555-561 (1980).
    Slot, C. Scand. J. Clin. Lab. Invest. 17, 381-387 (1965).
    Heinegard, D. and Tiderstroem, G. Clinica. Chimica. Acta. 43, 305-310 (1973).
    Cook, J.G.H. Ann. Clin. Biochem. 12, 219-232 (1975).