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Amyloid beta, 1-42, oligomeric, Stabilized Peptide
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Catalog Number
PE-1750
- Product Name Amyloid beta, 1-42, oligomeric, Stabilized Peptide
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Product Description
A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al., 2012, followed by lyophilisation using Biosensis' proprietary stabilization procedures.
The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric Aβ1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assay. This product is not recommended for western blots or other additional applications. - Alternative Names Beta-APP42; Beta-amyloid protein 42; ABPP; APPI; Amyloid beta A4 protein; AB42; abeta
- Application(s) ELISA, Other applications NOT recommended
- Purity Description Purified
- Regulatory Status For research use only.
Product Info
-
Product Description
A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al., 2012, followed by lyophilisation using Biosensis' proprietary stabilization procedures.
The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric Aβ1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assay. This product is not recommended for western blots or other additional applications. -
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Amyloid beta, 1-42, HFIP-treated Purified Peptide
- Application(s) ELISA, Other applications NOT recommended
-
Application Details
Use as positive control in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.
Use as oligomeric A-beta peptide standard in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click here for detailed instructions on generating a calibration curve with PE-1750-1000.
It is recommended to reconstitute the vial with 100 - 200 µL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions as directed above. Not recommended for western blots or other applications. - Target Amyloid beta, oligomeric
- Target Host Species Human
- Antigen Produced In Synthetic
- Sequence DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
- Purity Description Purified
- Appearance Lyophilized powder, without preservatives. May appear wet due to the hygroscopic nature of the stabilizing buffer, which does not affect product quality.
- Format Lyophilized, Supplied as 2 x 500 ng vials, each containing lyophilized Aβ oligomers. Note that the amount of provided oligomeric protein is based on the amount of monomeric Aβ used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.
- Storage Instructions Store unopened, lyophilized oligomeric Aβ with desiccant, insulated, at -20°C short term, -80°C long term. Store reconstituted vial at 2-8°C for up to 2 days. The reconstituted material should not be frozen for best results.
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt (unopened vial).
- Alternative Names Beta-APP42; Beta-amyloid protein 42; ABPP; APPI; Amyloid beta A4 protein; AB42; abeta
- Uniprot Number P05067
- Uniprot Number/Name P05067 (A4_HUMAN)
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41105330
- Regulatory Status For research use only.
Specifications
- General References Youmans KL. et al. (2012) Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody. Mol Neurodegener. 2012 Mar 16;7(1):8.