Casein, bovine beta A2, Purified Protein

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Catalog Number
PE-1832

    Product Info

  • Product Name Casein, bovine beta A2, Purified Protein
  • Product Description google
    PE-1832 is A2 beta-casein protein that is purified from bovine A2/A2 milk to >95% purity. The milk is collected from commercial cows certified free of disease and BSE by the manufacturer. Furthermore, because of the harsh acid/base purification conditions necessary to purify this protein from milk, the protein’s physical state is likely denatured, but the actual denaturation state has not been clearly determined, limiting the use of this protein solely to denatured western blot applications.
    IMPORTANT: Due to its formulation, this product may give different OD readings compared to the protein calibrator contained in our A2 Casein ELISA Kit. Thus, it is NOT recommended for use as a standard or QC marker in the A2 ELISA assays.
  • Alternative Names Beta Casein; CSN2
  • Application(s) WB, Other applications NOT recommended
  • Purity Description >95% (SDS-PAGE); Affinity-purified protein via antibody-mediated purification.
  • Purity % > 95%
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    PE-1832 is A2 beta-casein protein that is purified from bovine A2/A2 milk to >95% purity. The milk is collected from commercial cows certified free of disease and BSE by the manufacturer. Furthermore, because of the harsh acid/base purification conditions necessary to purify this protein from milk, the protein’s physical state is likely denatured, but the actual denaturation state has not been clearly determined, limiting the use of this protein solely to denatured western blot applications.
    IMPORTANT: Due to its formulation, this product may give different OD readings compared to the protein calibrator contained in our A2 Casein ELISA Kit. Thus, it is NOT recommended for use as a standard or QC marker in the A2 ELISA assays.
  • Related Products A1 Beta casein (A1), Bovine, ELISA assay
    A2 Beta casein (A2), Bovine, ELISA assay
    Casein, A2 beta, Chicken Polyclonal Antibody
    Casein, A1 beta, Chicken Polyclonal Antibody
    Casein, bovine beta A1, Purified Protein
    Beta casein protein, Rabbit Polyclonal Antibody
  • Application(s) WB, Other applications NOT recommended
  • Application Details

    Denatured, reduced Western blotting (100 ng or less) as a standard only. This material is not recommended for ELISA or ANY other application. Other applications have not been tested.

    Please follow the western blot resuspension and handling notes in the instructions included below for the best results:

    In reduced SDS_PAGE gels, beta caseins migrate at approximately 24-26kDa. Note that different gel conditions can cause beta caseins to migrate slower than normal, sometimes as high as 33-35kDa. The recommended gel is 4-20% Bis-Tris Gel with MOPS as a running buffer. For testing raw milk samples, stock milk must be first diluted. Dilute raw milk in 0.1M NaOH at 1:50. Then mix 10ul of diluted milk with 10µl 2X SDS-PAGE (with DTT) sample buffer and boil for 5 minutes, spin, and load 10µl/lane. For purified A1 or A2 protein, mix 10µl of prewarmed and mixed protein from the unopened vial with 10µl 2X SDS-PAGE (with DTT) sample buffer, boil for 5 minutes, spin, and load 8µl/lane.

    Note on handling: Purified beta caseins can and will precipitate out of the solution when cold or below pH 8. Before use in a western blot, the unopened vials should be warmed to 37°C, given a quick spin, and gently mixed with a pipette tip or vortex to ensure the precipitated protein has been redissolved.

    Purified Caseins are very sensitive to acidic conditions. At pH under pH8, they will easily precipitate or aggregate. The user is advised to keep this in mind when working with these proteins. Purified caseins are insoluble in neutral water. They do solubilize in alkali salts such as sodium acetate or sodium oxalate at pH readings over pH 8. Care in handling and storing these purified proteins is advised.

  • Target Casein, beta A2
  • Target Host Species Bovine
  • Antigen Produced In Natural A2/A2 Cows
  • Sequence Bovine A2 beta casein differs from A1 beta casein at amino acid position 82 (P82)
  • Purity Description >95% (SDS-PAGE); Affinity-purified protein via antibody-mediated purification.
  • Purity % > 95%
  • Format Liquid. Protein is provided as 50 µL aliquot containing protease inhibitor at 0.4 mg/mL in TBS, pH 8.5. Does not contain preservatives. Use sterile methods when handling. Tris-based buffers are subject to pH changes with temperature. Keep protein solutions at pH 8.0-8.5 for best results.
  • Storage Instructions Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -10°C to -20°C. Do not store at -80°C. Purified proteins will precipitate from the solution when cold or at a pH below 8.0. Store working aliquots short-term at 2-8°C as an undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS Purified Caseins are very sensitive to acidic conditions. At pH under pH8, they will easily precipitate or aggregate. The user is advised to keep this in mind when working with these proteins. Purified caseins are insoluble in neutral water. They do solubilize in alkali salts such as sodium acetate or sodium oxalate at pH readings over pH 8. Care in handling & storing these purified proteins is advised.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Beta Casein; CSN2
  • Uniprot Number P02666
  • Uniprot Number/Name P02666 (CASB_BOVIN)
  • Scientific Background The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.

    According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.

    A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.

    A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).

    Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.

    Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 12352202
  • Regulatory Status For research use only.

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