Product DescriptionL-selectin-Fc Chimera, Human, Purified Recombinant Protein, suitable to Block.
Application(s)Block
TargetL-selectin
Target Host SpeciesHuman
Antigen Produced InHEK293
SequenceA DNA sequence encoding the signal peptide, pro-peptide and extracellular domain of human L-Selectin (amino acids 1-343) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.
Purity Description>95%, as determined by SDS-PAGE and visualized by silver stain
Purity %> 95%
FormatThe L-Selectin-Fc chimera consists of 20-45% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution InstructionsReconstitute with sterile-filtered phosphate-buffered saline to a concentration of 100 µg/mL.
Storage InstructionsLyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundL-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites.
Lane 1: MW marker Lane 2: L-selectin-Fc Chimera (10 µg) Lane 3: L-selectin-Fc Chimera (10 µg) treated with PNGase F to remove potential N-linked glycans