SequenceA DNA sequence encoding the signal peptide and extracellular domain of human NGF receptor (amino acids 1-224) was fused to the Fc region of human IgG1 (amino acids 104-330). The chimeric protein was expressed in modified CHO cells. The full sequence is available on request from [email protected].
Purity Description>95%, as determined by SDS-PAGE.
Purity %> 95%
FormatFreeze-dried.
Reconstitution InstructionsIt is recommended that 0.25 mL of sterile phosphate-buffered saline (PBS) be added to the vial.
Storage InstructionsLyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundNerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity all members of the neurotrophin family including beta NGF, BDNF, NT-3 and NT-4/5. It also binds the pro-neurotrophins. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The p75NTR NGF receptor is a type I transmembrane glycoprotein (396 aa) consisting of a signal peptide (21 aa), an extracellular domain (225 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (19 aa) and a cytoplasmic domain (152 aa). It is a member of the TNF-alpha receptor family (TNR16). Recently, p75NTR has been shown to act as a receptor for many pathogens such as prions, rabies virus and amyloid beta. It also acts as a co-receptor for NOGO, mediating inhibitory signals of myelin associated protein.