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Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein
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Catalog Number
PE-1235
- Product Name Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein
- Product Description Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable for IP and to Block.
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2; Trkb; TrkB-Fc Chimera
- Application(s) Block, IP
- Purity Description >95%, as determined by SDS-PAGE and visualized by silver stain.
- Purity % > 95%
- Regulatory Status For research use only.
Product Info
- Product Description Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable for IP and to Block.
- Application(s) Block, IP
- Application Details The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Tyrosine Kinase Receptor B (TrkB)
- Target Host Species Human
- Antigen Produced In HEK293
- Sequence A DNA sequence encoding the signal peptide and extracellular domain of human TrkB (amino acids 1- 428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.
- Purity Description >95%, as determined by SDS-PAGE and visualized by silver stain.
- Purity % > 95%
- Format The chimera consists of 20-40% carbohydrate by weight.
- Reconstitution Instructions Reconstitute with sterile-filtered phosphate-buffered saline to a concentration of 20 µg/mL.
- Storage Instructions Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2; Trkb; TrkB-Fc Chimera
- Uniprot Number Q16620
- Uniprot Number/Name Q16620 (NTRK2_HUMAN)
- Scientific Background Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 12352202
- Regulatory Status For research use only.
Specifications
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Specific References
Beros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Application: Rat, Block.
Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis. Application: Rat, Block.