Product DescriptionRibosome-inactivating Purified Protein saporin-6 (Saporin), Purified Native Purified Protein, suitable for WB.
Application(s)WB
TargetRibosome-inactivating protein saporin-6 (Saporin)
Target Host SpeciesPlant
Antigen Produced InNative
Purity Description> 95% by SDS-PAGE. Purified from seeds of the plant Saponaria officinalis.
Purity %> 95%
FormatLyophilized
Reconstitution InstructionsSaporin may be reconstituted in the buffer of choice for example PBS.
Storage InstructionsKeep lyophilized protein at 2-8ºC. Aliquot and keep at -20°C for long-term storage. For short term keep aliquots at 2-8ºC. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSaponaria officinalis; Common soapwort; ribosome inactivating protein saporin-6; SAP-6; SO-6; rRNA N-glycosidase
Scientific BackgroundSaporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. Biosensis Saporin is purified in-house and available in bulk reuqest upon request as well.