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Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain for identifying Normal & Pathogenic Myelin

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Catalog Number

TR-100-B

Product Info

Product Name Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain for identifying Normal & Pathogenic Myelin
Product Description google Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.

Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Alternative Names BlackGold, Black and Gold
Application(s) IHC-Frozen, IHC-non-Paraffin-embedded
Specificity Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system.
Species Reactivity Mammals
Concentration 10X
Purity Description Purified
Regulatory Status For research use only.

Specifications

Product Description Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination.

Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Application(s) IHC-Frozen, IHC-non-Paraffin-embedded
Application Details Black Gold II is a high resolution myelin stain with amyloid plaque counter stain. Its use is tailored to studies using formalin or paraformaldehyde fixed, non-paraffin embedded, non-solvent processed brain tissue. It can be used with both thick and thin sections. For thick sections, gelatin coated slides or slides specially designed to bind tissues sections should be use to avoid section loss. Free-floating sections can be used as well but sections are easier to handle and transfer when mounted on slides. A suggested method for thick sections is provided as a guide: Either frozen or vibratome sections are cut at a thickness of 20-50 μm and collected in 0.1 M neutral phosphate buffer. The sections are then typically mounted on 1% gel-coated slides and then air dried on a slide warmer (at 50°C) for at least an hour until throughly dried and adhered to the slide. The sections can be stained loose, although the sections are easier to handle when mounted on slides. The mounted sections were rehydrated in distilled water for 2 minutes before being processed in the staining solutions.
Target Normal & pathogenic myelin
Specificity Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system.
Target Host Species Species Independent
Species Reactivity Mammals
Detection Method Colorimetric
Kit Components Black-Gold II (Dilute 1:10 prior to use) - 10 mL
Sodium Thiosulfate, fixative (Dilute 1:10 prior to use) - 10 mL
Toluidine Blue O (Dilute 1:10 prior to use) - 10 mL
Acetic Acid (Dilute 1:10 prior to use) - 10 mL
Purity Description Purified
Format The reagents in the Black Gold kit (10X) are all supplied in a liquid format and are ready-to-dilute.
Concentration 10X
Storage Instructions The kit can be transported at room temperature. Once received, the kit canbe stored for up to 12 months at 2-8°C protected from light. Diluted solutionscan be stored up to one month at 2-8°C protected from light.
Batch Number Please see item label.
Expiration Date Unopened kit 6 months at 2-8ºC protected from light. See Storage instructions for working solutions recommendations.
Alternative Names BlackGold, Black and Gold
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 60103920
Regulatory Status For research use only.

Images, Protocols & SDS

Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluadine Blue O stained polymorph cells (centre) and granule cells (top). Photo is courtesy of Dr. Larry Schmued.
Bright field illumination (60X mag) of 8 month old Ad-Tg mouse hippocampus. The myelin pathology can be observed in and around amyloid plaques. The cell bodies of adjacent granule and polymorph cells appear blue, while individual myeliated fibres appear nearly black. Photo is courtesy of Dr. Larry Schmued.

Human brain Locus K (Del Fiacco et al., Brain Struct Funct. 2014, 2019: 2083-2101; Cells 2018, 7, 72): calcitonin gene-related peptide-like immunoreactivity (black) and myelin staining (red, Black-Gold II staining kit, Biosensis) in the medulla oblongata, showing the similar neurochemical and structural organization of the dorsal column nuclei Locus K and the spinal trigeminal substantia gelatinosa.
Kit Protocol
TR-100-BG_as_at_March2020.pdf
SDS Link
SDS_TR-100-BG_as_at_Dec2021.pdf

Citations & References

Specific References Xing YL et al. (2023) High-efficiency pharmacogenetic ablation of oligodendrocyte progenitor cells in the adult mouse CNS. Cell Rep. [Epub ahead of print]

Deng S et al. (2022) Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination. Adv Sci (Weinh). [Epub ahead of print]

Díaz MM et al. (2022) DCC/netrin-1 regulates cell death in oligodendrocytes after brain injury. Cell Death Differ. [Epub ahead of print]

Lee J et al. (2022) PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous system. Life Sci Alliance. [Epub ahead of print]

Germundson DL & Nagamoto-Combs K. (2022) Potential Role of Intracranial Mast Cells in Neuroinflammation and Neuropathology Associated with Food Allergy. Cells. 11(4):738.

Kihara Y et al. (2021) Ponesimod inhibits astrocyte-mediated neuroinflammation and protects against cingulum demyelination via S1P1-selective modulation. FASEB J. 36(2):e22132.

Toomey LM et al. (2021) Cuprizone feed formulation influences the extent of demyelinating disease pathology. Sci Rep. 11(1):22594.

Del Fiacco M et al. (2018) TRPV1-Like Immunoreactivity in the Human Locus K, a Distinct Subregion of the Cuneate Nucleus. Cells. 2018 Jul 8;7(7). pii: E72.

Ying YL et al. (2014) Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination. J Neurosci. 2014 Oct 15;34(42):14128-46