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Prostate-specific antigen (PSA), Human, ELISA assay
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Catalog Number
BEK-2199
- Product Name Prostate-specific antigen (PSA), Human, ELISA assay
- Product Description The human Kallikrein-3 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Kallikrein-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Kallikrein-3 concentration are added to these wells and allowed to complex with the bound Kallikrein-3 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Kallikrein-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Kallikrein-3 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; P-30; Semenogelase; KLK3; APS; Kallikrein-3;
- Application(s) ELISA
- Specificity Human Kallikrein-3 E
- Species Reactivity Human
- Immunogen Description The antigen and standard used in this Kallikrein-3 ELISA is recombinant human Kallikrein-3 amino acids 18-261 produced in and purified from a mammalian cell expression system.
- Range 312 pg/mL - 20,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Kallikrein-3 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Kallikrein-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Kallikrein-3 concentration are added to these wells and allowed to complex with the bound Kallikrein-3 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Kallikrein-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Kallikrein-3 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Prostate-specific antigen (PSA) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Prostate-specific antigen (PSA)
- Specificity Human Kallikrein-3 E
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The antigen and standard used in this Kallikrein-3 ELISA is recombinant human Kallikrein-3 amino acids 18-261 produced in and purified from a mammalian cell expression system.
- Range 312 pg/mL - 20,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; P-30; Semenogelase; KLK3; APS; Kallikrein-3;
- Uniprot Number P07288
- Uniprot Number/Name P07288 (KLK3_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.